国产精品国产三级国产aⅴ原创,国产一级a,久久天天,亚洲av日韩av高潮潮喷无码

您好,歡迎進入上海力敏實業(yè)有限公司網站!
一鍵分享網站到:
您現在的位置:首頁 >> 產品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> sigma現貨代理Dispase II D-4693

sigma現貨代理Dispase II D-4693

  • 更新時間:  2023-07-26
  • 產品型號:  
  • 簡單描述
  • sigma現貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細介紹

sigma現貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現貨,和選購


留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
色亚州成人| 一呦二呦三呦精品网站| 极品午夜| 欧美专区日韩| 最新亚洲网站| 人妻性爱无码中文字幕| 国产精品视频区| 日韩欧美成人18| 久久久久人妻| 欧美91| 色悠悠网站免费观看| 人妻中出无码| 伊人玖玖综合网| 黄色网站国产精品| 人妻无码中文字幕免费视频蜜桃| 中文三级av| 日韩精品无码专区蜜桃| 无码 足取| 性按摩一区二区三区| 国产精品自拍99视频| 色呦呦免费123| 999中文字幕激情| 涩涩蜜桃视频www| 五月婷婷丁香亚洲| 红五月丁香啪啪啪啪| 亚州色图www| 日本视频网站在线观看| 国产蜜芽麻之一区二区| 国产精品呻吟高潮久久无码| 在线区| 天天干天天日夜夜操| 91人妻出轨| 久久黄色网络| 麻豆Xxx网站| 国产亚洲精品自拍。| 一二三四中国无码高清| 日日干夜夜爽夜夜操| 国产美女被插入| 国产亚洲日本精品一区| 啊啊啊好舒服免费| 亚洲熟妇久久国内精品|